pMSCV-Nras61K-Puro
(Plasmid #49404)

• Purpose: MSCVpuro based vector, expressing Nras mutant 61K.
• Depositing Lab: Tobias Neff
• Publication: MSCV-Nras61K-Puro (unpublished)

Backbone

• Vector backbone: pMSCV-puro
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6700
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N Ras K61
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Mutation: 61K constitutively activated
• GenBank ID: NM_002524
• Entrez Gene: NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
• Promoter: MSCV promotor

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bglii (destroyed during cloning)
• 3′ cloning site: Bglii (destroyed during cloning)
• 5′ sequencing primer: MSCV puro
• 3′ sequencing primer: MSCV puro

Resource Information

• Supplemental Documents:
  • Nras
• A portion of this plasmid was derived from a plasmid made by: Plasmid 12543: pBabe N-Ras 61K from Der Channing
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry