pBlue-TAL
(Plasmid
#49401)
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Purpose(Empty Backbone) The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript KS+
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Modifications to backboneSynthetic 2030 bp DNA fragment encoding truncated TAL-effector N- and C-terminus and FokI nuclease domain was subcloned into a pBluescript KS+ plasmid digested with Pvu II.
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Vector typeInsect Expression, TALEN
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Selectable markerslacZ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional reference: The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitfly. Takasu Y. et al. Methods 2014. PMID: 24565747
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBlue-TAL was a gift from Michal Zurovec (Addgene plasmid # 49401 ; http://n2t.net/addgene:49401 ; RRID:Addgene_49401) -
For your References section:
Efficient TALEN Construction for Bombyx mori Gene Targeting. Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, Uchino K, Sezutsu H, Tamura T, Zurovec M. PLoS One. 2013 Sep 18;8(9):e73458. doi: 10.1371/journal.pone.0073458. 10.1371/journal.pone.0073458 PubMed 24058473
Map uploaded by the depositor.
