pLP-hSox2-ires-gfp
(Plasmid #49391)

• Purpose: mammalian bicistronic expression of human Sox2 and EGFP
• Depositing Lab: Randall McKinnon
• Publication: McKinnon Lab plasmids (unpublished)

Backbone

• Vector backbone: pLP-IRES2-EGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression, Cre/Lox ; acceptor vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sox2
• Alt name: human Sry
• Species: H. sapiens (human)
• Insert Size (bp): 953
• Mutation: TGA (stop) to CCA (Pro) at nt 1027 with respect to GenBank ID BC013923
• GenBank ID: BC013923
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHN (C terminal on backbone)
  • IRES (C terminal on backbone)
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.