Skip to main content
Addgene

pDNR-hSox2
(Plasmid #49389)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDNR-dual
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Cre/Lox ; donor vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox2
  • Alt name
    human Sry
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    953
  • Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect to GenBank ID BC013923
  • GenBank ID
    BC013923
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)
  • Promoter none
  • Tag / Fusion Protein
    • 6xHN (6 repeating His-Asn subunits) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer CAT-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

from ATCC #10658153

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDNR-hSox2 was a gift from Randall McKinnon (Addgene plasmid # 49389 ; http://n2t.net/addgene:49389 ; RRID:Addgene_49389)
Related items:
Commonly requested with: