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Addgene

pDNR-hAkt1
(Plasmid #49387)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49387 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDNR-dual
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Cre/Lox ; donor vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Akt1
  • Alt name
    v-akt murine thymoma viral oncogene homolog 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1487
  • Mutation
    tga to cca at nt 1725 with respect to GenBank ID BC000479
  • GenBank ID
    BC000479
  • Entrez Gene
    AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Promoter none
  • Tag / Fusion Protein
    • 6xHN (6 repeating His-Asn subunits) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer CAT-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDNR-hAkt1 was a gift from Randall McKinnon (Addgene plasmid # 49387 ; http://n2t.net/addgene:49387 ; RRID:Addgene_49387)