pDNR-hAkt1
(Plasmid
#49387)
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Purposedonor vector for cloning human Akt1 with 6xHN tag
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDNR-dual
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4900
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Vector typeCre/Lox ; donor vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAkt1
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Alt namev-akt murine thymoma viral oncogene homolog 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1487
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Mutationtga to cca at nt 1725 with respect to GenBank ID BC000479
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GenBank IDBC000479
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter none
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Tag
/ Fusion Protein
- 6xHN (6 repeating His-Asn subunits) (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer CAT-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDNR-hAkt1 was a gift from Randall McKinnon (Addgene plasmid # 49387 ; http://n2t.net/addgene:49387 ; RRID:Addgene_49387)
Map uploaded by the depositor.