pcDNA3 Connexin43-GFP-APEX2
(Plasmid #49385)

• Purpose: Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells
• Depositing Lab: Alice Ting
• Publication: Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5366
• Total vector size (bp): 8039
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Connexin43 - EGFP - APEX2
• Species: soybean
• Insert Size (bp): 2673
• Mutation: K14D, W41F, E112K, A134P on soybean APX; improved for proteomics and EM applications
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHrev

Resource Information

• Supplemental Documents:
  • APEX2 Recommendations from Ting Lab
• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry