pGLB2 5′ mouse CNS
(Plasmid #49348)

• Purpose: luciferase reporter with mouse Bmp2 promoter and 3' conserved noncoding sequence (CNS)
• Depositing Lab: Melissa Rogers
• Publication: Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8. Epub 2004 Sep 9.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5598
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Bmp2 distal promoter
• Alt name: Bmp2
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Entrez Gene: Bmp2 (a.k.a. Bmp2a)
• Promoter: -
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: LucNrev

Gene/Insert 2

• Gene/Insert name: Bmp2 3' CNS (conserved noncoding sequence)
• Alt name: Bmp2
• Alt name: ultra-conserved sequence (UCS)
• Species: M. musculus (mouse)
• Insert Size (bp): 630
• Entrez Gene: Bmp2 (a.k.a. Bmp2a)
• Promoter: -

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: PvuII (destroyed during cloning)
• 3′ cloning site: SacI (destroyed during cloning)
• 5′ sequencing primer: LucF

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' insert contains nt –1,237 to 471 relative to distal transcription start.
3' insert contains nt 9574-10204 relative to distal transcription start and does not contain BmpA polyA sites (only has SV40 polyA site from vector).
Also called pGLB2–5′3′CNS.