pGL3 2198
(Plasmid #49347)

• Purpose: This is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.
• Depositing Labs: Stephen Harris, Melissa Rogers
• Publication: Feng et al Biochim Biophys Acta. 1997 Jan 3;1350(1):47-52.

Backbone

• Vector backbone: pGL3 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bmp2 promoter (shorter sequence)
• Alt name: pGL3 2185
• Species: H. sapiens (human)
• Entrez Gene: BMP2 (a.k.a. BDA2, BMP2A, SSFSC, SSFSC1)
• Promoter: Bmp2 promoter
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: NA
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry