pGLB2 5'3'
(Plasmid #49346)

• Purpose: luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)
• Depositing Lab: Melissa Rogers
• Publication: Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8. Epub 2004 Sep 9.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5598
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Bmp2 distal promoter
• Alt name: Bmp2
• Alt name: Construct B
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Entrez Gene: Bmp2 (a.k.a. Bmp2a)
• Promoter: -
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: LucNrev

Gene/Insert 2

• Gene/Insert name: Bmp2 3' UTR
• Alt name: Bmp2
• Species: M. musculus (mouse)
• Insert Size (bp): 2215
• Entrez Gene: Bmp2 (a.k.a. Bmp2a)
• Promoter: -

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: StyI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: LucF

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' region contains nt –1,237 to 471 relative to distal transcription start.
3' region contains nt 9574-11604 relative to distal transcription start and contains two Bmp2 polyadenylation signals yielding 3’UTRs of 870 and 1185 nt (Liu et al. PMID 18703506) and downstream flanking sequence.