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Addgene

pGL1.7XX
(Plasmid #49344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmp2 distal promoter
  • Alt name
    Bmp2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1702
  • Mutation
    nt –1237 to +471
  • GenBank ID
    12156
  • Entrez Gene
    Bmp2 (a.k.a. Bmp2a)
  • Promoter Bmp2 distal promoter
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL1.7XX was a gift from Melissa Rogers (Addgene plasmid # 49344 ; http://n2t.net/addgene:49344 ; RRID:Addgene_49344)

  • For your References section:

    An evolutionary and molecular analysis of Bmp2 expression. Abrams KL, Xu J, Nativelle-Serpentini C, Dabirshahsahebi S, Rogers MB. J Biol Chem. 2004 Apr 16;279(16):15916-28. Epub 2004 Feb 2. 10.1074/jbc.M313531200 PubMed 14757762
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