Skip to main content
Addgene

pJM126
(Plasmid #49339)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET11a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 9300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    RFA3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    730
  • Entrez Gene
    RFA3 (a.k.a. YJL173C, RPA14, RPA3)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RFA2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1100
  • Entrez Gene
    RFA2 (a.k.a. YNL312W, BUF1, RPA2, RPA32)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    RFA1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1900
  • Entrez Gene
    RFA1 (a.k.a. YAR007C, BUF2, FUN3, RPA1, RPA70)
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer NA
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJM126 was a gift from Steven Brill (Addgene plasmid # 49339 ; http://n2t.net/addgene:49339 ; RRID:Addgene_49339)

  • For your References section:

    Assessing the requirements for nucleotide excision repair proteins of Saccharomyces cerevisiae in an in vitro system. He Z, Wong JM, Maniar HS, Brill SJ, Ingles CJ. J Biol Chem. 1996 Nov 8;271(45):28243-9. 10.1074/jbc.271.45.28243 PubMed 8910442
Related items:
Commonly requested with: