pCS2+TA
(Plasmid
#49338)
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PurposepCS2+ vector with MCS replaced by a TA-cloning linker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Total vector size (bp) 4836
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Modifications to backbonethe multiple cloning site was replaced with a TA-cloning linker containing EGFP
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Vector typeGateway compatible cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTA-cloning linker containing EGFP
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Insert Size (bp)790
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Sp6
- 3′ sequencing primer m13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+TA was a gift from Heather Verkade (Addgene plasmid # 49338 ; http://n2t.net/addgene:49338 ; RRID:Addgene_49338)