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Addgene

pME-TA
(Plasmid #49337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME vector
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 3331
  • Modifications to backbone
    TA-cloning linker containing EGFP has been added between the attB sites.
  • Vector type
    Gateway cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TA-cloning linker containing EGFP
  • Insert Size (bp)
    790

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AleI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer m13F
  • 3′ sequencing primer m13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-TA was a gift from Heather Verkade (Addgene plasmid # 49337 ; http://n2t.net/addgene:49337 ; RRID:Addgene_49337)
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