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PurposepHAGE NFKB-TA-LUC-UBC-dTomato-W
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE
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Backbone manufacturerRichard Mulligan
- Backbone size w/o insert (bp) 4874
- Total vector size (bp) 9486
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameNFkB consensus binding sequence
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Insert Size (bp)66
Gene/Insert 2
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Gene/Insert nameTA
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Alt nameMinimal TA promoter
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Insert Size (bp)7
Gene/Insert 3
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Gene/Insert nameLuciferase
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Insert Size (bp)1653
Gene/Insert 4
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Gene/Insert nameUBC
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Alt nameUbiquitin-C Promoter
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Insert Size (bp)411
Gene/Insert 5
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Gene/Insert namedTomato
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Insert Size (bp)1431
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE NFKB-TA-LUC-UBC-dTomato-W was a gift from Darrell Kotton (Addgene plasmid # 49335 ; http://n2t.net/addgene:49335 ; RRID:Addgene_49335) -
For your References section:
Lentiviral delivery of RNAi for in vivo lineage-specific modulation of gene expression in mouse lung macrophages. Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN. Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013 Feb 12. 10.1038/mt.2013.19 PubMed 23403494