CaV1.3e[8a,11,31b,Δ32,42a]
(Plasmid #49333)

• Purpose: Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
• Depositing Lab: Diane Lipscombe
• Publication: Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Backbone

• Vector backbone: pcDNA6/V5-His ABC
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10F'
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CACNA1D
• Alt name: calcium channel, voltage-dependent, L type, alpha 1D subunit
• Alt name: Cav1.3
• Species: R. norvegicus (rat)
• Insert Size (bp): 5700
• Mutation: Contains exons 8a, 11, 31b and 42a.Deletion of exon 32.
• Entrez Gene: Cacna1d
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Isolated from superior cervical ganglia.

For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm