MSCV-myrAkt
(Plasmid #49267)

• Purpose: retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
• Depositing Labs: Lawrence Kane, Arthur Weiss
• Publication: Kane et al Nat Immunol. 2001 Jan;2(1):37-44.

Backbone

• Vector backbone: MSCV 2.2 IRES GFP
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt
• Alt name: AKT1
• Species: B. taurus (bovine)
• Insert Size (bp): 1500
• Mutation: N2D, D149E and A478G compared to AAW71957.1
• Entrez Gene: AKT1 (a.k.a. BOS_20453)
• Tags / Fusion Proteins:
  • myr (N terminal on insert)
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pLXSN-5
• 3′ sequencing primer: pCDH-rev (GCATTCCTTTGGCGAGAG)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified N2D, D149E and A478G mutations in the Akt sequence in this plasmid when compared to GenBank reference sequence AAW71957.1. These mutations were not intentional and the plasmid functions as described in the associated publication (i.e. it is constitutively active).