MSCV-Eya2
(Plasmid
#49265)
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Purposeretroviral expression of Eya2 and YFP
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV 2.2 IRES GFP
- Backbone size w/o insert (bp) 6500
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Modifications to backboneGFP replaced with YFP
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEya2
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SpeciesH. sapiens (human)
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Entrez GeneEYA2 (a.k.a. EAB1)
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Tags
/ Fusion Proteins
- IRES-YFP (C terminal on backbone)
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pLXSN 5'
- 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG) (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-Eya2 was a gift from Heide Ford (Addgene plasmid # 49265 ; http://n2t.net/addgene:49265 ; RRID:Addgene_49265)