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Addgene

pcDNA5-Flag-EAF1
(Plasmid #49249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EAF1
  • Alt name
    ELL associated Factor 1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_033083.6
  • Entrez Gene
    EAF1
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-Flag-EAF1 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 49249 ; http://n2t.net/addgene:49249 ; RRID:Addgene_49249)

  • For your References section:

    Human mediator subunit MED26 functions as a docking site for transcription elongation factors. Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE, Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW. Cell. 2011 Jul 8;146(1):92-104. 10.1016/j.cell.2011.06.005 PubMed 21729782
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