pIRES2-EGFP-deltaNp53:WTp53
(Plasmid #49244)

• Purpose: expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells
• Depositing Lab: Dylan Taatjes
• Publication: Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi: 10.1111/acel.12108. Epub 2013 Jun 25.

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone manufacturer: clonetch
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: p53 deltaN and p53 WT
• Alt name: TP53
• Species: H. sapiens (human)
• Insert Size (bp): 2343
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Promoter: CMV
• Tags / Fusion Proteins:
  • His (C terminal on insert)
  • IRES-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences are not known to affect function, though they may affect diagnostic digests.