pNIC28-hKdm2b-CxxC-PHD
(Plasmid #49216)

• Purpose: Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.
• Depositing Lab: Rob Klose
• Publication: Klose Lab BioCAP plasmids (unpublished)

Backbone

• Vector backbone: pNIC28
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 5290
• Total vector size (bp): 5866
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Kdm2b
• Species: H. sapiens (human)
• Insert Size (bp): 576
• Entrez Gene: KDM2B (a.k.a. CXXC2, FBXL10, Fbl10, JHDM1B, PCCX2)
• Promoter: T7
• Tags / Fusion Proteins:
  • His6-TEV (N terminal on insert)
  • Avi (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SGC
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry