pEF‐PIK3IP1
(Plasmid #49214)

• Purpose: expresses PIK3IP1 in mammalian cells
• Depositing Lab: Lawrence Kane
• Publication: DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. doi: 10.1002/eji.201141653. Epub 2012 Jul 19.

Backbone

• Vector backbone: pEF1/myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIK3IP1
• Alt name: phosphoinositide-3-kinase interacting protein 1
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Entrez Gene: Pik3ip1 (a.k.a. RP23-191E3.6, 1500004A08Rik, 5830455E04Rik, Crkd, Hgfl)
• Promoter: EF1a
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • 6X-his (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: BGH_rev_primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry