Flag‐Tim2
(Plasmid #49213)

• Purpose: expresses Flag tagged Tim2
• Depositing Lab: Lawrence Kane
• Publication: Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

Backbone

• Vector backbone: pCDEF3-CD8SS-Flag
• Backbone manufacturer: Kane Lab
• Modifications to backbone: a pCDEF3 expression plasmid containing the human CD8α signal sequence and Flag epitope tag
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tim2
• Alt name: T cell immunoglobulin and mucin domain containing 2
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Mutation: no Tim2 signal sequence; R80L and S196D compared to NP_599010.4
• Entrez Gene: Timd2 (a.k.a. G9D3, TIM-2, Tim2)
• Promoter: EF1a
• Tags / Fusion Proteins:
  • CD8 signal sequence (N terminal on backbone)
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EF1a-F
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry