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Addgene

Flag‐Tim2
(Plasmid #49213)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDEF3-CD8SS-Flag
  • Backbone manufacturer
    Kane Lab
  • Modifications to backbone
    a pCDEF3 expression plasmid containing the human CD8α signal sequence and Flag epitope tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tim2
  • Alt name
    T cell immunoglobulin and mucin domain containing 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Mutation
    no Tim2 signal sequence; R80L and S196D compared to NP_599010.4
  • Entrez Gene
    Timd2 (a.k.a. G9D3, TIM-2, Tim2)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • CD8 signal sequence (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag‐Tim2 was a gift from Lawrence Kane (Addgene plasmid # 49213 ; http://n2t.net/addgene:49213 ; RRID:Addgene_49213)

  • For your References section:

    Cutting edge: inhibition of T cell activation by TIM-2. Knickelbein JE, de Souza AJ, Tosti R, Narayan P, Kane LP. J Immunol. 2006 Oct 15;177(8):4966-70. PubMed 17015678
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