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pCDEF3‐hTIM3 (no tag)
(Plasmid #49212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDEF3
  • Backbone size w/o insert (bp) 6094
  • Total vector size (bp) 6940
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tim3
  • Alt name
    hepatitis A virus cellular receptor 2
  • Alt name
    Tim-3
  • Alt name
    Havcr2
  • Species
    H. sapiens (human)
  • Entrez Gene
    HAVCR2 (a.k.a. CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3)
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDEF3‐hTIM3 (no tag) was a gift from Lawrence Kane (Addgene plasmid # 49212 ; http://n2t.net/addgene:49212 ; RRID:Addgene_49212)

  • For your References section:

    Phosphotyrosine-dependent coupling of Tim-3 to T-cell receptor signaling pathways. Lee J, Su EW, Zhu C, Hainline S, Phuah J, Moroco JA, Smithgall TE, Kuchroo VK, Kane LP. Mol Cell Biol. 2011 Oct;31(19):3963-74. doi: 10.1128/MCB.05297-11. Epub 2011 Aug 1. 10.1128/MCB.05297-11 PubMed 21807895
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