Flag‐mTim3 (T1 truncation)
(Plasmid
#49210)
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Purposeexpresses Flag tagged mouse Tim3 (T1 truncation)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDEF3-CD8SS-Flag
- Total vector size (bp) 7012
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Modifications to backbonea pCDEF3 expression plasmid containing the human CD8α signal sequence and Flag epitope tag
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTim3 T1 truncation
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Alt namehepatitis A virus cellular receptor 2
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Alt nameHAvcr2
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Alt nameTim-3
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SpeciesM. musculus (mouse)
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MutationT1 truncation ( contains all but the three most C-terminal tyrosines); no Tim3 signal sequence
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Entrez GeneHavcr2 (a.k.a. TIM-3, Tim3, Timd3)
- Promoter EF1a
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Tags
/ Fusion Proteins
- signal sequence (N terminal on backbone)
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag‐mTim3 (T1 truncation) was a gift from Lawrence Kane (Addgene plasmid # 49210 ; http://n2t.net/addgene:49210 ; RRID:Addgene_49210) -
For your References section:
Phosphotyrosine-dependent coupling of Tim-3 to T-cell receptor signaling pathways. Lee J, Su EW, Zhu C, Hainline S, Phuah J, Moroco JA, Smithgall TE, Kuchroo VK, Kane LP. Mol Cell Biol. 2011 Oct;31(19):3963-74. doi: 10.1128/MCB.05297-11. Epub 2011 Aug 1. 10.1128/MCB.05297-11 PubMed 21807895