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Addgene

Flag‐mTim3 (del.Cyto)
(Plasmid #49209)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49209 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDEF3-CD8SS-Flag
  • Total vector size (bp) 6872
  • Modifications to backbone
    a pCDEF3 expression plasmid containing the human CD8α signal sequence and Flag epitope tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tim3 (del.Cyto)
  • Alt name
    hepatitis A virus cellular receptor 2
  • Alt name
    HAvcr2
  • Alt name
    Tim-3
  • Species
    M. musculus (mouse)
  • Mutation
    cytosolic domain deleted, no Tim3 signal sequence; Y218F compared to NP_599010.4
  • Entrez Gene
    Havcr2 (a.k.a. TIM-3, Tim3, Timd3)
  • Promoter Ef1a
  • Tags / Fusion Proteins
    • CD8 signal sequence (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag‐mTim3 (del.Cyto) was a gift from Lawrence Kane (Addgene plasmid # 49209 ; http://n2t.net/addgene:49209 ; RRID:Addgene_49209)

  • For your References section:

    Phosphotyrosine-dependent coupling of Tim-3 to T-cell receptor signaling pathways. Lee J, Su EW, Zhu C, Hainline S, Phuah J, Moroco JA, Smithgall TE, Kuchroo VK, Kane LP. Mol Cell Biol. 2011 Oct;31(19):3963-74. doi: 10.1128/MCB.05297-11. Epub 2011 Aug 1. 10.1128/MCB.05297-11 PubMed 21807895
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