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Purposeexpresses Flag-tagged human Tim1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDEF3-CD8SS-Flag
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Backbone manufacturerKane Lab
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTim1
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Alt namehepatitis A virus cellular receptor 1
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Alt nameHavcr1
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SpeciesH. sapiens (human)
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MutationTim1 lacks its own signal sequence
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Entrez GeneHAVCR1 (a.k.a. CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1)
- Promoter EF1a
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Tags
/ Fusion Proteins
- signal sequence (N terminal on backbone)
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cDNA PCR amplified from Integrated Molecular Analysis of Genomes and their Expression (I.M.A.G.E.) consortium clone #3826378
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag‐hTim1 was a gift from Lawrence Kane (Addgene plasmid # 49207 ; http://n2t.net/addgene:49207 ; RRID:Addgene_49207) -
For your References section:
T cell Ig and mucin 1 (TIM-1) is expressed on in vivo-activated T cells and provides a costimulatory signal for T cell activation. de Souza AJ, Oriss TB, O'malley KJ, Ray A, Kane LP. Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17113-8. Epub 2005 Nov 11. 10.1073/pnas.0508643102 PubMed 16284246