pCDEF3‐mTim1 (no tag)
(Plasmid
#49206)
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Purposeexpresses mouse Tim1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDEF3
- Backbone size w/o insert (bp) 6094
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTim1
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Alt namehepatitis A virus cellular receptor 1
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Alt nameHavcr1
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SpeciesM. musculus (mouse)
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Entrez GeneHavcr1 (a.k.a. RP23-31K14.2, AI503787, KIM-1, TIM-1, Tim1, Timd1)
- Promoter EF1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDEF3‐mTim1 (no tag) was a gift from Lawrence Kane (Addgene plasmid # 49206 ; http://n2t.net/addgene:49206 ; RRID:Addgene_49206) -
For your References section:
T cell Ig and mucin 1 (TIM-1) is expressed on in vivo-activated T cells and provides a costimulatory signal for T cell activation. de Souza AJ, Oriss TB, O'malley KJ, Ray A, Kane LP. Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17113-8. Epub 2005 Nov 11. 10.1073/pnas.0508643102 PubMed 16284246