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Addgene

Flag‐mTim1 (Bl/6)
(Plasmid #49205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDEF3-CD8SS-Flag
  • Backbone manufacturer
    Kane Lab
  • Modifications to backbone
    a pCDEF3 expression plasmid containing the human CD8α signal sequence and Flag epitope tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tim1 (BL/6 allele)
  • Alt name
    hepatitis A virus cellular receptor 1
  • Alt name
    Havcr1
  • Species
    M. musculus (mouse)
  • Mutation
    Tim1 lacks its own signal sequence
  • Entrez Gene
    Havcr1 (a.k.a. RP23-31K14.2, AI503787, KIM-1, TIM-1, Tim1, Timd1)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • signal sequence (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag‐mTim1 (Bl/6) was a gift from Lawrence Kane (Addgene plasmid # 49205 ; http://n2t.net/addgene:49205 ; RRID:Addgene_49205)

  • For your References section:

    T cell Ig and mucin 1 (TIM-1) is expressed on in vivo-activated T cells and provides a costimulatory signal for T cell activation. de Souza AJ, Oriss TB, O'malley KJ, Ray A, Kane LP. Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17113-8. Epub 2005 Nov 11. 10.1073/pnas.0508643102 PubMed 16284246
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