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Addgene

pPCR-Script-mLIP
(Plasmid #49194)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPCR-Script Amp SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 3450
  • Vector type
    Bacterial Expression ; cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C/EBPbeta (LIP fragment)
  • Alt name
    CCAAT/enhancer binding protein (C/EBP), beta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    463
  • Mutation
    only the LIP fragment
  • Entrez Gene
    Cebpb (a.k.a. C/EBPbeta, CRP2, IL-6DBP, LAP, LIP, NF-IL6, NF-M, Nfil6)
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPCR-Script-mLIP was a gift from Jed Friedman (Addgene plasmid # 49194 ; http://n2t.net/addgene:49194 ; RRID:Addgene_49194)
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