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Addgene

pFA6a-12Pk-ura4MX6
(Plasmid #49180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Vector type
    Yeast Expression
  • Selectable markers
    URA4
  • Tag / Fusion Protein
    • 12Pk (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are two adenines in Addgene's T7 quality control sequence that introduce a HindIII site and an AflII site into the plasmid. Note that these discrepancies with the depositor sequence do NOT affect the function of the plasmid. The vector is used as a template for PCR to amplify DNA cassettes to modify chromosomal gene loci. The additional adenines are not within the primer sequences to amplify DNA cassettes for gene manipulation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-12Pk-ura4MX6 was a gift from Eishi Noguchi (Addgene plasmid # 49180 ; http://n2t.net/addgene:49180 ; RRID:Addgene_49180)

  • For your References section:

    New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100. 10.2144/000114100 PubMed 24215641
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