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Purpose(Empty Backbone) genomic 12Pk epitope tagging in S. pombe
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
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Vector typeYeast Expression
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- 12Pk (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-12Pk-hphMX6 was a gift from Eishi Noguchi (Addgene plasmid # 49177 ; http://n2t.net/addgene:49177 ; RRID:Addgene_49177) -
For your References section:
New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100. 10.2144/000114100 PubMed 24215641
Map uploaded by the depositor.
