GABA(A) receptor subunit B3SE
(Plasmid #49171)

• Purpose: pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
• Depositing Labs: Tija Jacob, Stephen Moss
• Publication: Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

Backbone

• Vector backbone: modified pRK5
• Backbone manufacturer: BD PharMingen
• Total vector size (bp): 7097
• Modifications to backbone: region 5’ of the cloning site is slightly different
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GABA(A) receptor subunit beta 3
• Alt name: Gabrb3
• Species: M. musculus (mouse)
• Mutation: GTG (V) to TGC (C)
• GenBank ID:
• Entrez Gene: Gabrb3 (a.k.a. A230092K12Rik, Cp1, Gabrb-3, beta3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • pHGFP (pHluorin) (N terminal on insert)
  • myc (N terminal on insert)
  • thrombin cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: P5

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Deletion of YK at end of pHGFP, added extra base to keep frame before myc-tag