GABA(A) receptor subunit g2SE
(Plasmid #49170)

• Purpose: pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
• Depositing Labs: Tija Jacob, Stephen Moss
• Publication: Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

Backbone

• Vector backbone: modified pRK5
• Backbone manufacturer: BD PharMingen
• Total vector size (bp): 7557
• Modifications to backbone: region 5’ of the cloning site is slightly different
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GABA(A) receptor subunit gamma 2
• Alt name: Gabrg2
• Species: M. musculus (mouse)
• GenBank ID:
• Entrez Gene: Gabrg2 (a.k.a. GABAA-R, Gabrg-2, gamma2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • myc (N terminal on insert)
  • pHGFP (pHluorin) (N terminal on insert)
  • thrombin cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: P5

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

myc and pHGFP are inserted into coding sequence of gamma2 subunit.
R to T mutation (AGG-ACG) does not affect function.