GABA(A) receptor subunit a1SE
(Plasmid #49168)

• Purpose: pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
• Depositing Labs: Tija Jacob, Stephen Moss
• Publication: Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. doi: 10.1523/JNEUROSCI.5050-07.2008.

Backbone

• Vector backbone: modified pRK5
• Backbone manufacturer: BD PharMingen
• Total vector size (bp): 7837
• Modifications to backbone: region 5’ of the cloning site is slightly different
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GABA(A) receptor subunit alpha-1
• Alt name: GBRA1
• Species: M. musculus (mouse)
• GenBank ID: P62812
• Entrez Gene: Gabra1 (a.k.a. GABAA-alpha1, GABAAR-alpha1, Gabra-1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • myc (N terminal on insert)
  • pHGFP (pHIuorin) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: P5

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: myc-pHIuorin are inserted into coding sequence of alpha 1 subunit.