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Addgene

ME2/pBABE-puro
(Plasmid #49164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Addgene plasmid # 1764
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 6924
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Malic Enzyme 2
  • Alt name
    ME2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1755
  • Entrez Gene
    ME2 (a.k.a. ODS1)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer pBABE-5
  • 3′ sequencing primer pBABE-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ME2/pBABE-puro was a gift from Xiaolu Yang (Addgene plasmid # 49164 ; http://n2t.net/addgene:49164 ; RRID:Addgene_49164)

  • For your References section:

    Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Jiang P, Du W, Mancuso A, Wellen KE, Yang X. Nature. 2013 Jan 31;493(7434):689-93. doi: 10.1038/nature11776. Epub 2013 Jan 13. 10.1038/nature11776 PubMed 23334421
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