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Addgene

pLJM1-STAG2
(Plasmid #49162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49162 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLJM1
  • Backbone manufacturer
    David Sabatini
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 11200
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow in XL10 Gold or Stbl2 cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3696
  • GenBank ID
    NM_001042751.1
  • Entrez Gene
    STAG2 (a.k.a. SA-2, SA2, SCC3B, bA517O1.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1-STAG2 was a gift from Todd Waldman (Addgene plasmid # 49162)

  • For your References section:

    Frequent truncating mutations of STAG2 in bladder cancer. Solomon DA, Kim JS, Bondaruk J, Shariat SF, Wang ZF, Elkahloun AG, Ozawa T, Gerard J, Zhuang D, Zhang S, Navai N, Siefker-Radtke A, Phillips JJ, Robinson BD, Rubin MA, Volkmer B, Hautmann R, Kufer R, Hogendoorn PC, Netto G, Theodorescu D, James CD, Czerniak B, Miettinen M, Waldman T. Nat Genet. 2013 Oct 13. doi: 10.1038/ng.2800. 10.1038/ng.2800 PubMed 24121789
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