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Addgene

pSmart-Nm-sgRNA-BbsI Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb plus ladder and a digestion of the plasmid pSmart-Nm-sgRNA-BbsI with the following enzymes: HindIII-HF and EcoRI-HF.

Expected Bands
HindIII-HF: 1025 bp, 791 bp, & 420 bp
EcoRI-HF: 1776 bp & 460 bp

We observe the expected bands for this digest.