Setd3-Flag-pGEX4t-1
(Plasmid
#49145)
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Purposebacterial expression of GST-Setd3-Flag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49145 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSetd3
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SpeciesM. musculus (mouse)
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Insert Size (bp)1800
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Entrez GeneSetd3 (a.k.a. 2610102I01Rik, 2610305M23Rik, 6030490B17Rik, D12Ertd771e)
- Promoter tac
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Setd3-Flag-pGEX4t-1 was a gift from Jing H Wang (Addgene plasmid # 49145 ; http://n2t.net/addgene:49145 ; RRID:Addgene_49145) -
For your References section:
The role of a newly identified SET domain-containing protein, SETD3, in oncogenesis. Chen Z, Yan CT, Dou Y, Viboolsittiseri SS, Wang JH. Haematologica. 2013 May;98(5):739-43. doi: 10.3324/haematol.2012.066977. Epub 2012 Oct 12. 10.3324/haematol.2012.066977 PubMed 23065515
