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Addgene

pEMS2118
(Plasmid #49143)

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Item Catalog # Description Quantity Price (USD)
Plasmid 49143 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    AAV2
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ssAAV-smCBA-iCre-WPRE
  • Alt name
    iCre
  • Species
    Synthetic
  • Promoter smCBA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2118 was a gift from Elizabeth Simpson (Addgene plasmid # 49143 ; http://n2t.net/addgene:49143 ; RRID:Addgene_49143)

  • For your References section:

    rAAV-PHP.B escapes the mouse eye and causes lethality whereas rAAV9 can transduce aniridic corneal limbal stem cells without lethality. Mirjalili Mohanna SZ, Korecki AJ, Simpson EM. Gene Ther. 2023 Apr 19. doi: 10.1038/s41434-023-00400-6. 10.1038/s41434-023-00400-6 PubMed 37072572
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