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Addgene

pEMS2114
(Plasmid #49139)

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Item Catalog # Description Quantity Price (USD)
Plasmid 49139 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    AAV2
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ssAAV-Ple67-emGFP
  • Alt name
    emGFP
  • Species
    Synthetic
  • Promoter FEV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2114 was a gift from Elizabeth Simpson (Addgene plasmid # 49139 ; http://n2t.net/addgene:49139 ; RRID:Addgene_49139)

  • For your References section:

    Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM. Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. 10.1534/genetics.119.301984 PubMed 30765420
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