pEMS1990
(Plasmid #49121)

• Purpose: AAV plasmid with FEV (Ple67) promoter driving expression of iCre.
• Depositing Lab: Elizabeth Simpson
• Publication: Korecki et al Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984.

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Backbone

• Vector backbone: AAV2
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ssAAV-Ple67-iCre
• Alt name: iCre
• Species: Synthetic
• Promoter: FEV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pEMS1990 Genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.