pEMS1982
(Plasmid #49113)

• Purpose: AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.
• Depositing Lab: Elizabeth Simpson
• Publication: Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10.1016/j.exer.2019.107728.

Backbone

• Vector backbone: AAV2
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ssAAV-Ple261-iCre
• Alt name: iCre
• Species: Synthetic
• Promoter: CLDN5

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pEMS1982 Genbank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.

Please see the following publication for more information:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye.
de Leeuw et al. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. PMID 27164903