pEF5B-FRT-AP-Sstr3-GFP-DEST
(Plasmid #49098)

• Purpose: Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines
• Depositing Lab: Maxence Nachury
• Publication: Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013.

Backbone

• Vector backbone: pEF5B
• Backbone size w/o insert (bp): 6833
• Total vector size (bp): 7240
• Modifications to backbone: Gateway Cloning DEST vector from Life Technologies
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach T1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sstr3
• Alt name: somatostatin receptor 3
• Species: M. musculus (mouse)
• Insert Size (bp): 1283
• Entrez Gene: Sstr3 (a.k.a. Smstr-3, Smstr3, sst3)
• Promoter: T7
• Tags / Fusion Proteins:
  • acceptor peptide (AP) (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: AT GGG CCT GAA CGA CAT C
• 3′ sequencing primer: CT TGT ACA GCT CGT CCA TGC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SSTR3-GFP was a gift from Dr. Kirk Mykytyn
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry