CS2+MYC-Etv5:T139D
(Plasmid
#49097)
-
Purposeexpression of Etv5 T139D mutant
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 49097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCs2+
-
Backbone manufacturerRZPD
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 5659
-
Vector typeMammalian Expression ; xenopus/avian/zebrafish
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameEtv5 T139D
-
Alt nameerm
-
Alt nameEtv5
-
SpeciesD. rerio (zebrafish)
-
MutationT139D
-
Entrez Geneetv5a (a.k.a. DKEY-189G17.1, etv5, fj87h10, id:ibd5133, wu:fj87h10, zgc:55647)
- Promoter CMV
-
Tag
/ Fusion Protein
- MYC (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CS2+MYC-Etv5:T139D was a gift from Michael Tsang (Addgene plasmid # 49097 ; http://n2t.net/addgene:49097 ; RRID:Addgene_49097) -
For your References section:
Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development. Znosko WA, Yu S, Thomas K, Molina GA, Li C, Tsang W, Dawid IB, Moon AM, Tsang M. Dev Biol. 2010 Jun 1;342(1):11-25. Epub 2010 Mar 24. 10.1016/j.ydbio.2010.03.011 PubMed 20346941