pDULE-ABK
(Plasmid #49086)

• Purpose: A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
• Depositing Lab: Peter Schultz
• Publication: Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7. doi: 10.1002/cbic.201100194. Epub 2011 Jun 15.

Backbone

• Vector backbone: pDule
• Backbone size w/o insert (bp): 6290
• Total vector size (bp): 7590
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: tRNA synthetase
• Species: M. barkeri
• Mutation: L274M, C313A, Y349F
• Promoter: lpp

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: tRNA for a photocrosslinking amino acid
• Species: Synthetic
• Promoter: GlnRS

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: NA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry