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PurposeMammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 49082 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGW1
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Backbone manufacturerBritish Biotechnology
- Backbone size w/o insert (bp) 5270
- Total vector size (bp) 7010
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePercevalHR
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SpeciesSynthetic
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Insert Size (bp)1740
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GW1-PercevalHR was a gift from Gary Yellen (Addgene plasmid # 49082 ; http://n2t.net/addgene:49082 ; RRID:Addgene_49082) -
For your References section:
Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Tantama M, Martinez-Francois JR, Mongeon R, Yellen G. Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. 10.1038/ncomms3550 PubMed 24096541
Map uploaded by the depositor.
