pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)
(Plasmid #49077)

• Purpose: Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
• Depositing Lab: Biff Forbush
• Publication: Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi: 10.1371/journal.pone.0082060.

Backbone

• Vector backbone: pcDNA3.1 hygro
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5484
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human NKCC1 (synthetic)
• Alt name: Slc12A2 (synthetic)
• Alt name: Na-K-Cl cotransporter
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 4506
• Mutation: 262 silent mutations from native hNKCC1
• Entrez Gene: SLC12A2 (a.k.a. BSC, BSC-2, BSC2, CCC1, KILQS, NKCC1, PPP1R141)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3x HA (N terminal on insert)
  • mCerulean (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Synthetic cDNA encoding full length human NKCC1, with 29 silent restriction sites in NKCC1 coding region