pBABE-ddIPpoI
(Plasmid #49052)

• Purpose: Expresses the restriction enzyme under the control of Shield1
• Depositing Lab: Michael Kastan
• Publication: Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Backbone

• Vector backbone: pBABE
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DD-HA-ER-IPpoI
• Species: H. sapiens (human); Physarum polycephalum
• Insert Size (bp): 1826

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry