pET-15b-EK_C122S_His5
(Plasmid #49048)

• Purpose: Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli
• Depositing Lab: Hans Brandstetter
• Publication: Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi: 10.1016/j.jbiotec.2013.10.022. Epub 2013 Oct 30.

Backbone

• Vector backbone: pET-15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5708
• Total vector size (bp): 6392
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enteropeptidase, peptidase domain
• Alt name: Enterokinase
• Species: B. taurus (bovine)
• Mutation: P92R, E185D, C122S
• GenBank ID: NM_174439.2
• Entrez Gene: TMPRSS15 (a.k.a. PRSS7)
• Promoter: T7
• Tag / Fusion Protein:
  • 5xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Haidong Tan (Dalian Institute of Chemical Physics, PR China), see Tan et al., Protein Expr. Purif. 56 (2007), 40–47
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry